Journal: Bioengineering

Article Title: Focused Low-Intensity Pulsed Ultrasound (FLIPUS) Mitigates Apoptosis of MLO-Y4 Osteocyte-like Cells

doi: 10.3390/bioengineering10030387

Figure Lengend Snippet: FLIPUS enhances the metabolic activity of starved MLO-Y4 osteocytes, measured by WST-8 assay. FI to NC stands for fold induction over negative control, samples which did not receive stimulation with ultrasound. Each data point was the result of N = 3 and n = 3, where N and n are biological trials and technical replicates, respectively. ** and *** represent p ≤ 0.01 and p ≤ 0.001, respectively.

Article Snippet: WST-8 solution (BIOZOL DV GmbH, Eching, Germany) then was added to each well in a 1:10 ratio, and the cells were cultured in the incubator for another 30 min. Absorbance was measured at 450 nm with reference to 620 nm on a BMG LabTech FLUORstar OPTIMA reader (2015 series, Ortenberg, Germany).

Techniques: Activity Assay, Negative Control

Journal: Journal of the American Chemical Society

Article Title: OptoChaperoneA Biohybrid Tool for Regulating Protein Condensates in Cells and In Vitro

doi: 10.1021/jacs.6c04074

Figure Lengend Snippet: Effect of OptoChaperone on cell viability under heat treatment. (a) Schematic of cell viability assay. HeLa cells electroporated with OptoChaperone were seeded 96-well plates. After adherence, cells were irradiated for 5 min with UV (OFF) or blue (ON) light to switch the state of OptoChaperone immediately before heat treatment. Cells were then subjected to 120 min of heat treatment at 43 °C, followed by a 48 h incubation at 37 °C to assess delayed cytotoxicity. Cell viability was measured using a water-soluble tetrazolium 8 (WST-8) assay after 4 h of incubation with the reagent. (b) Quantitative analysis of cell viability after light irradiation and heat treatment. Viability values were normalized to those of HeLa cells without OptoChaperone and without heat treatment (set as 100%). The plots show each data points, and the mean ± s.d. ( N = 3). Statistical significance was determined by one-way ANOVA followed by Tukey’s HSD (n.s., not significant; * p < 0.05).

Article Snippet: Subsequently, cells were incubated at 37 °C and 5% CO 2 for 48 h to allow cell viability phenotypes to manifest, with fresh DMEM introduced after 24 h. After the 48 h incubation, 100 μL of DMEM (prewarmed to 37 °C) containing 10% v/v WST-8 reagent (Cat No. 260-96162; Kishida Chemical Co., Osaka, Japan) was added to each well.

Techniques: Viability Assay, Irradiation, Incubation